标准号：ISO/TS 16649-3-2005
实施状态：作废
中文名称：食品和动物饲料的微生物学.β葡糖苷酸酶阳性大肠杆菌记数的水平方法.第3部分:用5-溴-4-氯3-吲哚-β-D葡糖苷酸的莫斯随机计数技术
英文名称：Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Most probable number technique using 5-bromo-4-chloro-3-indolyl-<beta>-D-glucuronide
发布日期：2005-09
被替代标准：ISO 16649-3-2015
代替标准：ISO/DIS 16649-3-2004
采用标准：DIN ISO/TS 16649-3-2009,IDT;DIN ISO/TS 16649-3-2008,IDT;BS DD ISO/TS 16649-3-2005,IDT;XP V08-031-3-2005,IDT
起草单位：ISO/TC 34
标准简介：This Technical Specification specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 ℃, then at 44 ℃.
This Technical Specification is applicable to
— products intended for human consumption and the feeding of animals, and
— environmental samples in the area of food production and food handling.
The method is suitable for the enumeration of cells of Escherichia coli that may have been subjected to stress arising from dehydration, freezing, exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.
A limitation of the applicability of this Technical Specification is imposed by the susceptibility of the method to a large degree of variability. The method is to be applied and the results interpreted in the light of the information given in Clause 11.
WARNING — Strains of Escherichia coli that do not grow at 44 ℃ and, in particular, those that are β-glucuronidase negative, such as Escherichia coli O157 and some other strains of pathogenic E. coli, will not be detected by the method described in this Technical Specification.
文件格式：PDF
文件大小：194.22KB
文件页数：14
（以上信息更新时间为：2019-11-22）
ISO_TS 16649-3-2005 食品和动物饲料的微生物学.β葡糖苷酸酶阳性大肠杆菌记数的水平方法.第3部分_用5-溴-4-氯3-吲哚-β-D葡糖苷酸的莫斯随机计数技术.pdf
(194.22 KB)
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